A Methodology for Utilization of Predictive Genomic Signatures in FFPE Samples

ABSTRACT: Purpose: Gene expression signatures developed to measure the activity of oncogenic signaling pathways have been used to dissect the heterogeneity of tumor samples and to predict sensitivity to various cancer drugs that target components of the relevant pathways, thus potentially identifying therapeutic options for subgroups of patients. To facilitate broad use, including in a clinical setting, the ability to generate data from formalin-fixed, paraffin-embedded (FFPE) tissues is essential. Experimental Design: Patterns of pathway activity in matched fresh-frozen and FFPE xenograft tumor samples were generated using the MessageAmp Premier methodology in combination with assays using Affymetrix arrays. Results generated were compared with those obtained from fresh-frozen samples using a stan

ORGANISM(S): Homo sapiens

SUBMITTER: Holly Dressman 

PROVIDER: E-GEOD-29598 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress