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Continuous GH treatment of old rat liver

ABSTRACT: Effect of continuous GH treatment on old rat liver. Male rats, 2-year-old, were treated with vehicle or human GH (0.34 microgram/gram body weight) for 3 weeks. Comparison of treated and untreated rats. Individual RNA were used for 4 microarray hybridization. Dye swapped.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Amilcar Flores

PROVIDER: E-GEOD-2962 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Exploring hepatic hormone actions using a compilation of gene expression profiles.

Ståhlberg Nina N Merino Roxana R Hernández Luis Henríquez LH Fernández-Pérez Leandro L Sandelin Albin A Engström Pär P Tollet-Egnell Petra P Lenhard Boris B Flores-Morales Amilcar A

BMC physiology 20050613 1

<h4>Background</h4>Microarray analysis is attractive within the field of endocrine research because regulation of gene expression is a key mechanism whereby hormones exert their actions. Knowledge discovery and testing of hypothesis based on information-rich expression profiles promise to accelerate discovery of physiologically relevant hormonal mechanisms of action. However, most studies so-far concentrate on the analysis of actions of single hormones and few examples exist that attempt to use ...[more]

PMID: 15953391

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Continuous GH treatment of old rat liver

Project description:Effect of continuous GH treatment on old rat liver. Male rats, 2-year-old, were treated with vehicle or human GH (0.34 microgram/gram body weight) for 3 weeks. Keywords: response of old rat liver to growth hormone

2005-07-19 | GSE2962 | GEO

Glycopeptides from recombinant EBV gH, gL and gp42 with EThcD

Project description:Soluble ectodomains of Epstein Barr Virus glycoproteins gH/gL and gp42 were expressed in HEK293F cells for structural characterization by cryo electron microscopy. The purified material was additionally analyzed by LC-MS/MS after digestion with trypsin or chymotrypsin to identify N-linked glycosylation from EThcD glycopeptide fragmentation spectra.

2018-04-10 | PXD006403 | Pride

Genome-wide analyses reveal the extent of opportunistic STAT5 binding that does not yield transcriptional activation of neighboring genes.

Project description:Signal Transducers and Activators of Transcription (STAT) 5A/B regulate cytokine-inducible genes upon binding to GAS motifs. It is not known what percentage of genes with GAS motifs bind to and are regulated by STAT5. Moreover, it is not clear whether genome-wide STAT5 binding is modulated by its concentration. To clarify these issues we established genome-wide STAT5 binding upon growth hormone (GH) stimulation of wild type mouse embryonic fibroblasts (MEFs) and MEFs overexpressing STAT5A more than 20-fold. Upon GH stimulation 23,827 and 111,939 STAT5A binding sites were detected in wild type and STAT5A overexpressing MEFs, respectively. 13,278 and 71,561 peaks contained at least one GAS motif. 1,586 and 8,613 binding sites were located within 2.5 kbp of promoter sequences, respectively. Stringent filtering revealed 78 genes in which the promoter/upstream region (-10kbp to +0.5kbp) was recognized by STAT5 both in wt and STAT5 overexpressing MEFs and 347 genes that bound STAT5 only in overexpressing cells. Genome-wide expression analyses identified that the majority of STAT5-bound genes was not under GH control. Up to 40% of STAT5-bound genes were not expressed. For the first time we demonstrate the magnitude of opportunistic genomic STAT5 binding that does not translate into transcriptional activation of neighboring genes. Genome-wide mapping of STAT5 binding in MEF cells (WT, KO; Stat5-/- and overexpression; STAT5A-Stat5-/-) upon growth hormone induction

2012-01-12 | E-GEOD-34986 | biostudies-arrayexpress

Sex-dependent and growth hormone (GH)-dependent gene expression

Project description:A series of dual-channel gene expression profiles obtained using MWG Rat 5K microarrays (~5535 unique rat genes) and MWG Rat Liver microarrays (~1353 unique rat genes with 999 of these genes also represented on the Rat 5K microarray) was used to examine the sex-dependent and GH-dependent differences in gene expression in adult rat liver. This series is comprised of 8 randomly chosen pairings of independent male and female rat liver cDNA samples and 8 randomly chosen pairings of independent male and continuous GH-treated male rat liver cDNA samples, totaling 16 samples. Half of the samples were hybridized to the MWG Rat 5K microarrays and the other half were hybridized to the MWG Rat Liver microarrays. Comparison of the set of sex-dependent genes with the set of GH-responsive genes shows that 90% of male-dominant genes are suppressed in male rats treated with a female pattern of GH. Approximately 73% of female-dominant genes were up-regulated in the continuous GH-treated male rats. Keywords = Growth hormone Keywords = liver sexual dimorphism Keywords = cytochrome P450 Keywords = liver gene expression Keywords = dual channel cDNA microarray Keywords: repeat sample

2004-03-27 | GSE1146 | GEO

Response of liver-expressed genes (including lincRNAs) to continuous growth hormone (GH) infusion

Project description:Gene expression in livers of adult male mice subjected to continuous GH infusion using Alzet osmotic minipumps for 1, 4 or 7 days was assayed by RNA-seq, as part of a study of growth hormone regulation of hepatic lincRNAs (PMID:26459762) and protein-coding genes (PMID:28694329).

2015-10-21 | GSE66008 | GEO

Coho Salmon GH Transgenic Study

Project description:Coho Salmon GH Transgenic Study Keywords: other

2005-05-01 | GSE2388 | GEO

Project description:Mus musculus Gh, growth hormone [Source:MGI Symbol;Acc:MGI:95707], is differentially expressed in 35 experiment(s);

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Project description:Mus musculus Gh, growth hormone [Source:MGI Symbol;Acc:MGI:95707], is expressed in 11 baseline experiment(s);

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Project description:Ovis aries GH, growth hormone [Source:NCBI gene (formerly Entrezgene);Acc:443329], is expressed in 1 baseline experiment(s);

| ENSOARG00000013932 | ExpressionAtlas

Growth Hormone (GH) Treatment of 3T3-F442A Adipocytes

Project description:Expression profiling of 3T3-F442A adipocytes treated with growth hormone (GH, 500 nM) or vehicle (DMEM + 1% BSA) control for 30 min., 4 hr., or 48 hr in three independent experiments. Chronic GH treatment induces metabolic changes consistent with insulin resistance in 3T3-F442A adipocytes. Keywords: time-course

2005-12-20 | GSE2120 | GEO

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