Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Continuous GH treatment of old rat liver

ABSTRACT: Effect of continuous GH treatment on old rat liver. Male rats, 2-year-old, were treated with vehicle or human GH (0.34 microgram/gram body weight) for 3 weeks. Comparison of treated and untreated rats. Individual RNA were used for 4 microarray hybridization. Dye swapped.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Amilcar Flores

PROVIDER: E-GEOD-2962 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Exploring hepatic hormone actions using a compilation of gene expression profiles.

Ståhlberg Nina N Merino Roxana R Hernández Luis Henríquez LH Fernández-Pérez Leandro L Sandelin Albin A Engström Pär P Tollet-Egnell Petra P Lenhard Boris B Flores-Morales Amilcar A

BMC physiology 20050613 1

<h4>Background</h4>Microarray analysis is attractive within the field of endocrine research because regulation of gene expression is a key mechanism whereby hormones exert their actions. Knowledge discovery and testing of hypothesis based on information-rich expression profiles promise to accelerate discovery of physiologically relevant hormonal mechanisms of action. However, most studies so-far concentrate on the analysis of actions of single hormones and few examples exist that attempt to use ...[more]

PMID: 15953391

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Project description:Growth rate can be genetically modified in many vertebrates by domestication and selection, and more recently by transgenesis overexpressing growth factor genes (e.g. growth hormone, GH). While the phenotypic end consequence is similar, it is currently not clear whether the same modifications to physiological pathways are occurring in both genetic processes, nor to what extent they may interact when combined. To examine these questions, we have used rainbow trout as a model species because non-domesticated wild strains are available as comparators to assess genetic and physiological changes that have arisen from domestication and from GH transgenesis. In addition to pure wild and pure domesticated strains, two different GH transgenes with markedly different growth effects were examined, both in a wild background and in hybrids which combined domesticated and wild genomes in addition to the transgene. We find that liver mRNAs show highly concordant changes in levels in both types of fast-growing fish, relative to wild type, for both up- and down-regulated genes. Further, among domesticated, transgenic, and their hybrid genotypes, a strong positive correlation was found between growth rate and the number of genes affected or their levels of mRNA. Functional analysis found that genes involved in immune function, carbohydrate metabolism, detoxification, transcription regulation, growth regulation, and lipid metabolism were affected in common by domestication and GH transgenesis. The common responses of domesticated and GH transgenic strains is consistent with the GH pathway or its downstream effects being upregulated in domesticated animals during their modification from wild-type growth rates. Microarray analyses were performed on five individual rainbow trout per group of pure wild, pure domesticated, GH transgenic strain 1 in wild, GH transgenic strain 2 in wild, GH transgenic strain 1 in wild-domestic hybrid, and GH transgenic strain 2 in wild-domestic hybrid hybridized (one slide per individual) against a common wild-type RNA pool.

Dataset Information

Continuous GH treatment of old rat liver

Publications

Exploring hepatic hormone actions using a compilation of gene expression profiles.

Similar Datasets

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