Human embryonic stem cells derived from embryos at different stages of development share similar transcription profiles

ABSTRACT: We have derived hESC from biopsied blastomeres of cleavage stage embryos under virtually the same conditions we used for the derivation of hESC lines from inner cell mass of blastocyst stage embryos. Blastomere-derived hESC lines exhibited all the standard characteristics of hESC including undifferentiated proliferation, genomic stability, expression of pluripotency markers and the ability to differentiate into the cells of all three germ layers both in vitro and in vivo. To examine whether hESC lines derived from two developmental stages of the embryo differ in gene expression, we have subjected three blastomere-derived hESC lines and two ICM-derived hESC lines grown under identical culture conditions to transcriptome analysis using gene expression arrays. Unlike previously reported co

ORGANISM(S): Homo sapiens

SUBMITTER: Gnanaratnam Giritharan 

PROVIDER: E-GEOD-29625 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress