Postia placenta MAD-698 gene expression in ball-milled aspen or ball-milled pine medium

ABSTRACT: Transcript profiles of Postia placenta grown on media containing ball-milled aspen or ball-milled pine as the sole carbon source were analyzed. Array design was based on the DoE's Joint Genome Institute's gene models for P. placenta version 1. The research goal is to identtify genes essential for cellulose depolymerization. From a data set of 12,438 unique alleles, each NimbleGen (Madison, WI) array featured 10 unique 60mers per gene, all in triplicate. The dataset was manually annotated to include only the ‘best allelic model’ among CAZY-encoding genes. Total RNA was purified from medium containing ball-milled aspen or ball-milled pine as the sole carbon source. Three biological replicates per medium were used (6 separate arrays). RNA was converted to double-strand cDNA and labeled with

ORGANISM(S): Postia placenta

SUBMITTER: Dan Cullen 

PROVIDER: E-GEOD-29656 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress