Profiling of differential allelic expression in mouse placenta from reciprocal crosses

ABSTRACT: Many questions about the regulation, functional specialization, computational prediction, and evolution of genomic imprinting would be better addressed by having an exhaustive genome-wide catalog of genes that display parent-of-origin differential expression. As a first-pass scan for novel imprinted genes, we performed mRNA-seq experiments on E17.5 mouse placenta cDNA samples from reciprocal cross F1 progeny of AKR and PWD mouse strains, and quantified the allele-specific expression and the degree of parent-of-origin effect transcriptome-wide. We confirmed the imprinting status of 23 known imprinted genes in the placenta, and found that 12 genes reported previously to be imprinted in other tissues are also imprinted in mouse placenta. Through a well-replicated design using an orthogonal

ORGANISM(S): Mus musculus

SUBMITTER: Andrew Clark 

PROVIDER: E-GEOD-29690 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress