Effect of gibberellins on metabolism during rosette growth in Arabidopsis

ABSTRACT: To understand the effect of high and low GA levels on plant metabolism and development in Arabidopsis we made use of the GA biosynthesis inhibitor paclobutrazol (PBZ) and exogenously applied GA. The whole genome response at the translation level was assessed by immunopurification of polysomes from PBZ- and GA treated plants expressing FLAG-tagged ribosomal protein L18 (RPL18B). Polysomal associated RNA was isolated and subjected to affymterix ATH1 CHIP analysis. A total of 140 genes were statistically determined to be differentially translated after GA treatment whereas 89 genes where affected PBZ treatment. Our analysis revealed that GA and PBZ have opposing effects on the expression of cell wall and wax layer biosynthesis related genes. In addition, many genes involved in secondary meta

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Jos Schippers 

PROVIDER: E-GEOD-29699 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress