Gene expression analysis of host spleen responses to Marek's disease virus infection in susceptible and resistant chickens

ABSTRACT: To investigate gene expression profiles in susceptible spleens (SS) and resistant spleens (RS) from MDV-infected chickens and non-infected spleens (NS) from controls. Twelve spleens were chosen from three groups (four samples per group) to be used in chicken genome microarray to examine differential gene expression. In total, there were 4317 and 2593 differentially expressed (DE) genes in SS vs. NS and in SS vs. RS, respectively. However, no DE genes were found in RS vs. NS. The expression of twenty-four genes was verified by qPCR. In this study, we found weak, but detectable host immune response still existed in the late neoplastic transformation phase of MDV infection. Toll-like receptor pathway was affected in susceptible spleens compared to non-infected and resistant spleens. IL10 seem

ORGANISM(S): Gallus gallus

SUBMITTER: Ning Yang 

PROVIDER: E-GEOD-29805 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress