Global analysis of growth phase responsive gene expression and regulation of antibiotic biosynthetic pathways

ABSTRACT: The eubacterial species Streptomyces coelicolor proceeds through a complex growth cycle in which morphological differentiation/development is associated with a transition from primary to secondary metabolism and the production of antibiotics. We used DNA microarrays and mutational analysis to investigate the expression of individual genes and multigene antibiotic biosynthetic pathways during these events. We identified expression patterns in biosynthetic, regulatory, and ribosomal protein genes that were associated highly specifically with particular stages of development. A knowledge-based algorithm that correlates temporal changes in expression with chromosomal position identified groups of contiguous genes expressed at discrete stages of morphological development, inferred the boundarie

ORGANISM(S): Streptomyces coelicolor

SUBMITTER: Jane Huang 

PROVIDER: E-GEOD-2983 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress