Inferring drug-induced gene regulatory relationships in primary human hepatocytes

ABSTRACT: Statins are widely used cholesterol-lowering drugs that inhibit HMG-CoA reductase, a key enzyme in cholesterol synthesis. In some cases, however, these drugs may cause a number of toxic side effects in hepatocytes and skeletal muscle tissue. Currently, the specific molecular mechanisms that cause these adverse effects are not sufficiently understood. In this work, genome-wide RNA expression changes in primary human hepatocytes of six individuals were measured at five time points upon atorvastatin treatment. A novel systems-level analysis workflow was applied to reconstruct regulatory mechanisms based on these drug-response data and available knowledge about transcription factor binding specificities, protein-protein interactions and protein-drug interactions. Several previously unknown tra

ORGANISM(S): Homo sapiens

SUBMITTER: Michael Bonin 

PROVIDER: E-GEOD-29868 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress