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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Ezh2 and H3K27me3 in cardiomyocytes

ABSTRACT: This SuperSeries is composed of the following subset Series: GSE29992: Genome-wide profiling of E12.5 cardiomyocytes RNA expression in both hetozygeous control and mutant GSE29994: ChIP-seq of Ezh2 and H3K27me3 in E12.5 heart apex Refer to individual Series

ORGANISM(S): Mus musculus

SUBMITTER: Michael Hsing

PROVIDER: E-GEOD-29997 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Polycomb repressive complex 2 regulates normal development of the mouse heart.

He Aibin A Ma Qing Q Cao Jingjing J von Gise Alexander A Zhou Pingzhu P Xie Huafeng H Zhang Bing B Hsing Michael M Christodoulou Danos C DC Cahan Patrick P Daley George Q GQ Kong Sek Won SW Orkin Stuart H SH Seidman Christine E CE Seidman Jonathan G JG Pu William T WT

Circulation research 20111208 3

<h4>Rationale</h4>Epigenetic marks are crucial for organogenesis, but their role in heart development is poorly understood. Polycomb repressive complex 2 (PRC2) trimethylates histone H3 at lysine 27, which establishes H3K27me3 repressive epigenetic marks that promote tissue-specific differentiation by silencing ectopic gene programs.<h4>Objective</h4>We studied the function of PRC2 in murine heart development using a tissue-restricted conditional inactivation strategy.<h4>Methods and results</h4 ...[more]

PMID: 22158708

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ChIP-seq of Ezh2 and H3K27me3 in E12.5 heart apex

Project description:Ezh2 and H3K27me3 binding sites in E12.5 heart apex PRC2 binding sites in wild type cardiomyocytes

2012-03-15 | E-GEOD-29994 | biostudies-arrayexpress

Genome-wide profiling of E12.5 cardiomyocytes RNA expression in both heterozygous control and mutant

Project description:Congenital heart disease is among the most frequent major birth defects. Epigenetic marks are crucial for organogenesis, but their role in heart development is poorly understood. Polycomb Repressive Complex 2 (PRC2) trimethylates histone H3 at lysine 27, establishing H3K27me3 repressive epigenetic marks that promote tissue-specific differentiation by silencing ectopic gene programs. We studied the function of the catalytic subunit of PRC2, EZH2, in murine heart development. Early EZH2 inactivation by Nkx2-5Cre caused lethal congenital heart malformations, but slightly later EZH2 inactivation by cTNT-Cre did not. To study how the cardiomyocytes gene expression program is properly established in the early heart development, we combined the technologies of RNA sequencing and chromatin immunoprecipitation sequencing to identify the functional target genes directly repressed by EZH2. Intriguingly, these were enriched for transcriptional regulators of non-cardiac expression programs, such as transcription factors that regulate neuronal (Pax6) and cardiac progenitor genes (Isl1 and Six1). EZH2 was also required to maintain spatiotemporal regulation of cardiac gene expression, as Hcn4, Mlc2a, and Bmp10 were inappropriately upregulated in ventricular RNA. Furthermore, EZH2 was required for normal cardiomyocyte proliferation, establishing H3K27me3 epigenetic marks at cell cycle inhibitors Ink4a/b and repressing their expression. Our study reveals a previously undescribed role of EZH2 in regulating heart formation and shows that perturbation of the epigenetic landscape early cardiogenesis has sustained disruptive effects at later developmental stages. 8 E12.5 heart apex were used for RNA preparation each group.

2012-03-15 | E-GEOD-29992 | biostudies-arrayexpress

ChIP-seq of Ezh2 and H3K27me3 in E12.5 heart apex

Project description:Ezh2 and H3K27me3 binding sites in E12.5 heart apex

2012-03-15 | GSE29994 | GEO

Ezh2 and H3K27me3 in cardiomyocytes

Project description:This SuperSeries is composed of the SubSeries listed below.

2012-03-15 | GSE29997 | GEO

Interactome of the -subunit of L-type voltage-gated calcium channels in adult cardiomyocytes

Project description:In cardiomyocytes, Ca2+ influx through L-type voltage-gated calcium channels (LTCCs) following membrane depolarization regulates crucial Ca2+-dependent processes including duration and amplitude of the action potentials and excitation-contraction coupling. LTCCs are heteromultimeric proteins composed of the Cav1, Cav, Cav2 and Cav subunits. Here, using ascorbate peroxidase (APEX)-mediated proximity labeling and quantitative proteomics, we identified 61 proteins in the nano-environments of Cav2 in cardiomyocytes. These proteins are involved in diverse cellular functions such as cellular trafficking, muscular contraction, sarcomere organization and excitation-contraction coupling. Moreover, pull-down assays, co-immunoprecipitation analyses and super-resolution imaging using direct stochastic optical reconstruction microscopy (dSTORM) revealed that Cav2 interacts with the ryanodine receptor 2 (RyR2) in adult cardiomyocytes, probably coupling LTCCs and the RyR2 into a supramolecular complex at the dyads. This interaction is mediated by the Src-homology 3 domain of Cav2 and is necessary for an effective pacing frequency‐dependent increase of the Ca2+-induced Ca2+ release mechanism in cardiomyocytes.

2021-12-14 | PXD021375 | Pride

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Project description: Not available

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Cardiomyocytes microarray

Project description:Cardiomyocytes microarray

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Expression profiling analysis of differentiating human-induced pluripotent stem cell (hiPSC)-derived cardiomyocytes

Project description:This SuperSeries is composed of the following subset Series: GSE35671: Comparison of mRNA expression profiling of differentiating human-induced pluripotent stem cell (hiPSC)–derived cardiomyocytes, biopsies from fetal, adult and hypertensive human hearts and primary cardiomyocytes GSE35672: miRNA expression profiling of differentiating human-induced pluripotent stem cell (hiPSC)-derived cardiomyocytes Refer to individual Series

2012-02-10 | E-GEOD-35673 | biostudies-arrayexpress

Genome-wide gene expression analysis of Di-(2-ethyl hexyl) phthalate-treated cardiomyocytes

Project description:This SuperSeries is composed of the following subset Series: GSE21640: Gene expression analysis of cardiomyocytes treated with a clinically relevant concentration of DEHP (50 ug/mL) GSE21641: Dose-dependent gene expression analysis of cardiomyocytes treated with DEHP (1-50 ug/mL) Refer to individual Series

2010-09-01 | E-GEOD-21642 | biostudies-arrayexpress

Genome-wide gene expression analysis of new cardiomyocytes (CMs) derived from resident c-kitpos endogenous cardiac stem cells (eCSCs) after myocardial injury by Isoproterenol (ISO) in mice.

Project description:Genome-wide gene expression analysis of new cardiomyocytes (CMs) derived from resident c-kitpos endogenous cardiac stem cells (eCSCs) after myocardial injury by Isoproterenol (ISO) in mice. These data show that of new CMs derived from resident c-kitpos eCSCs have a gene expression profile that closely resembles the specific gene expression of adult cardiomyocytes. This result supports a role of c-kitpos eCSCs in the regeneration and repair of myocardial damage. To test the identity and the degree of differentiation of new cardiomyocytes (CMs) derived from resident c-kitpos endogenous cardiac stem cells (eCSCs) after myocardial injury by Isoproterenol (ISO) in mice. Global gene expression profiles by microarray was obtained in c-kitpos eCSCs, c-kitpos eCSC-derived YFPpos CMs and normal adult CMs. c-kitposCD45neg eCSCs were isolated from B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J mice and harvested at 4th passage (n=3). YFPpos cardiomyocytes were FACS sorted from left ventricle apex of Lentirius-c-kit/cre recombined B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J mice 28 days after ISO (N=3). Normal adult CMs were isolated from B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J mice (n=3).

2013-07-30 | E-GEOD-49318 | biostudies-arrayexpress

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