Reconstitution of the mouse germ-cell specification pathway in culture by pluripotent stem cells

ABSTRACT: The generation of properly functioning gametes in vitro, a key goal in developmental/reproductive biology, requires multi-step reconstitutions of complex germ cell development. Based on the logic of primordial germ cell (PGC)-specification, we demonstrate here the generation of PGC-like cells (PGCLCs) in mice with robust capacity for spermatogenesis from embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) through epiblast-like cells (EpiLCs), a cellular state highly similar to pre-gastrulating epiblasts, but distinct from epiblast stem cells (EpiSCs). The global transcription profiles, epigenetic reprogramming, and cellular dynamics during PGCLC induction from EpiLCs are a meticulous capture of those associated with PGC specification from the epiblasts. Furthermore, we i

ORGANISM(S): Mus musculus

SUBMITTER: Kazuki Kurimoto 

PROVIDER: E-GEOD-30056 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress