Identification of MVH target mRNA

ABSTRACT: During mammalian spermatogenesis the mouse VASA homolog (MVH), a germ cell-specific DEAD-box type RNA binding protein, localizes in a germline-specific RNA granule termed the chromatoid body (CB). Genetic analyses have revealed that MVH is essential to progress through spermatogenesis, although the molecular mechanisms of its function remain elusive. We found that the acetyltransferase HAT1 and its cofactor, p46, are specifically co-localized with MVH in the CB and acetylate MVH at Lys-405, leading to inactivation of its RNA binding activity. Notably, the acetylation is developmentally regulated, paralleling the temporally regulated co-localization of HAT1 and p46 in the CB. We have identified 858 mRNAs as MVH targets, a large proportion of which correspond to previously identified transla

ORGANISM(S): Mus musculus

SUBMITTER: Ippei Nagamori 

PROVIDER: E-GEOD-30144 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress