Digital gene expression (DGE) of CMV-infected tobacco leaves
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ABSTRACT: Here we performed a transcriptomic study on complete symptom development process of CMV-infected Nicotiana tabacum using Solexa/Illumina's high-throughput digital gene expression (DGE) system. 12 DGE libraries (from six virus-infected samples and six corresponding mock-inoculated samples) were constructed, and the gene expression variations between the virus-infected sample and the mock-inoculated sample in each symptom stage were compared. Thousands of differentially expressed genes were obtained by the comparison, and KEGG pathway analysis of these genes suggested that many biological processes (such as phtosynthesis, pigment metabolism and plant-pathogen interaction) were related to the symptom development. The authenticity of the DGE data was further confirmed by analyzing real-time RT
ORGANISM(S): Nicotiana tabacum
SUBMITTER: Jie Lu
PROVIDER: E-GEOD-30162 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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