Effect of 5-azacytidine on gene expression in C2C12 myoblasts

ABSTRACT: Mesenchymal progenitor cells can be differentiated in vitro into myotubes that exhibit many characteristic features of primary mammalian skeletal muscle fibers. However, in general, they do not show the functional excitation-contraction coupling or the striated sarcomere arrangement typical of mature myofibers. Epigenetic modifications have been shown to play a key role in regulating the progressional changes in transcription necessary for muscle differentiation. In this study, we demonstrate that treatment of murine C2C12 mesenchymal progenitor cells with 10 µM of the DNA methylation inhibitor 5-azacytidine (5AC) promotes myogenesis, resulting in myotubes with enhanced maturity as compared to untreated myotubes. Specifically, 5AC treatment resulted in the upregulation of muscle genes at t

ORGANISM(S): Mus musculus

SUBMITTER: Marlinda Hupkes 

PROVIDER: E-GEOD-30192 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress