DNA binding factors shape the mouse methylome at distal regulatory regions [ChIP_chip]

ABSTRACT: To gain insights into the interplay between DNA methylation and gene regulation we generated a basepair resolution reference map of the mouse methylome in stem cells and neurons. High genome coverage allowed for a novel quantitative analysis of local methylation states, which identified Low Methylated Regions (LMR) with an average methylation of 30%. These regions are evolutionary conserved, reside outside of CpG islands and distal to promoters. They represent regulatory regions evidenced by their DNaseI hypersensitivity and chromatin marks of enhancer elements. LMRs are occupied by transcription factors (TF) and their reduced methylation requires TF binding while introduction of TF binding sites creates LMRs de novo. This dependency on TF activity is further evident when comparing the met

ORGANISM(S): Mus musculus

SUBMITTER: Dirk Schuebeler 

PROVIDER: E-GEOD-30204 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress