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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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VirF gene from Shigella spp is able to induce chromosomal genes in E. coli

ABSTRACT: In order to understand whether the evolutionary acquisition of virF gene may have altered the transcriptional program in Shigella's harmless ancestor (E. coli) promoting the inactivation of genes potetially detrimental to the full expression of the invasivity phenotype, we performed a global transcriptional analysis of E. coli cells expressing or lacking the virF gene. To this end we used the E. coli K12 MG1655 strain, carrying the virF-encoding plasmid pMYSH6504, or its virF depleted derivative pMY6504R. Two condition experiment: the E. coli K12 MG1655 strain, carrying the virF-encoding plasmid pMYSH6504, and its virF depleted derivative pMY6504R. Two replicate for each condition

ORGANISM(S): Escherichia coli

SUBMITTER: Gianni Prosseda

PROVIDER: E-GEOD-30207 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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VirF gene from Shigella spp is able to induce chromosomal genes in E. coli

Project description:In order to understand whether the evolutionary acquisition of virF gene may have altered the transcriptional program in Shigella's harmless ancestor (E. coli) promoting the inactivation of genes potetially detrimental to the full expression of the invasivity phenotype, we performed a global transcriptional analysis of E. coli cells expressing or lacking the virF gene. To this end we used the E. coli K12 MG1655 strain, carrying the virF-encoding plasmid pMYSH6504, or its virF depleted derivative pMY6504R.

2011-06-25 | GSE30207 | GEO

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Project description:ChIPexo experiment was performed on E. coli K12 MG1655 with various supplements designed to activate transcriptional regulators

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Project description:RNA sequencing was performed on E. coli K12 MG1655 with various supplements designed to activate transcriptional regulators

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OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states

Project description:We performed evolution of Escherichia coli K12 MG1655 to study how the system adapt to iron toxicity. RNA-Seq was performed to examine the underlying transcriptional rewiring.

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Pseudogene repair driven by selection pressure applied in experimental evolution

Project description:We performed evolution of ∆menF∆entC strain of Escherichia coli K12 MG1655 to study how the system adapt to the loss of siderophore biosynthesis. RNA-Seq was performed to examine the underlying transcriptional rewiring.

2018-12-20 | GSE122779 | GEO

Project description:Study of plasmid stability in Escherichia coli K12 MG1655

| PRJNA534303 | ENA

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