Open chromatin defined by DNaseI and FAIRE identifies regulatory elements that shape cell-type identity [FAIRE_seq]

ABSTRACT: The human body contains thousands of unique cell types, each with specialized functions. Cell identity is governed in large part by gene transcription programs, which are determined by regulatory elements encoded in DNA. To identify regulatory elements active in seven cell lines representative of diverse human cell types, we used DNase-seq and FAIRE-seq to map M-CM-"M-BM-^@M-BM-^\open chromatinM-CM-"M-BM-^@M-BM-^]. Over 870,000 DNaseI or FAIRE sites, which correspond largely to nucleosome depleted regions (NDRs), were identified across the seven cell lines, covering nearly 9% of the genome. The combination of DNaseI and FAIRE is more effective than either assay alone in identifying likely regulatory elements, as judged by coincidence with transcription factor binding locations determined i

ORGANISM(S): Homo sapiens

SUBMITTER: Terry Furey 

PROVIDER: E-GEOD-30225 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress