Transcription profiling of human peripheral blood mononuclear cells, or whole blood were isolated 0, 30 min, 6 h, 24 h, and 7 day after intravenous endotoxin challenge vs controls
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
SUBMITTER: Anthony Suffredini
PROVIDER: E-GEOD-3026 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Gene expression profiles of peripheral blood leukocytes after endotoxin challenge in humans.
Talwar Shefali S Munson Peter J PJ Barb Jennifer J Fiuza Carmen C Cintron Anadel Pilar AP Logun Carolea C Tropea Margaret M Khan Sameena S Reda Debra D Shelhamer James H JH Danner Robert L RL Suffredini Anthony F AF
Physiological genomics 20060110 2
To define gene expression profiles that occur during the initial activation of human innate immunity, we administered intravenous endotoxin (n = 8) or saline (n = 4) to healthy subjects and hybridized RNA from blood mononuclear cells (0, 0.5, 6, 24, 168 h) or whole blood (0, 3, 6, 24, 168 h) to oligonucleotide probe arrays. The greatest change in mononuclear cell gene expression occurred at 6 h (439 induced and 428 repressed genes, 1% false discovery rate, and 50% fold change) including increase ...[more]
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