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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Tissue specific diurnal rhythm of transcripts and their regulation during herbivore attack in Nicotiana attenuata

ABSTRACT: Idenfication and characterization of oscillating transcripts after elicitation with oral secretions from the sepcialist herbivore, Manduca sexta larvae Source leaves, sink leaves and roots were collected every 4 h for one days.

ORGANISM(S): Nicotiana attenuata

SUBMITTER: Klaus Gase

PROVIDER: E-GEOD-30287 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Tissue specific diurnal rhythms of metabolites and their regulation during herbivore attack in a native tobacco, Nicotiana attenuata.

Kim Sang-Gyu SG Yon Felipe F Gaquerel Emmanuel E Gulati Jyotasana J Baldwin Ian T IT

PloS one 20111018 10

Ecological performance is all about timing and the endogenous clock that allows the entrainment of rhythms and anticipation of fitness-determining events is being rapidly characterized. How plants anticipate daily abiotic stresses, such as cold in early mornings and drought at noon, as well as biotic stresses, such as the timing of pathogen infections, is being explored, but little is known about the clock's role in regulating responses to insect herbivores and mutualists, whose behaviors are kn ...[more]

PMID: 22028833

Similar Datasets

NaJAZh regulates a subset of defense responses against herbivores and counteracts developmental necrosis in Nicotiana attenuata plants

Project description:Identification of transcripts that change expression in Nicotiana attenuata plants with reduced expression of JAZh after elicitation with oral secretions of Manduca sexta larvae. Gene expression in leaves of Nicotiana attenuata wild type and irJAZh plants was measured at 2 hour after elicitation with oral secretions of Manduca sexta larvae. Three independent experiments were performed with wild type plants and three independent experiments were performed with irJAZh plants. A total of 480 genes were identified as more the 2 fold differentially regulated in irJAZh plants compared to wild type plants.

2012-07-11 | E-GEOD-33681 | biostudies-arrayexpress

Characterization of Nicotiana attenuata plants with reduced expression of lectin receptor kinase 1 (ir-lecRK1 plants).

Project description:Identification of transcripts that change expression in Nicotiana attenuata plants with reduced expression of lectin receptor kinase 1 after elicitation with oral secretions of Manduca sexta larvae. Gene expression in leaves of Nicotiana attenuata wild type and ir-lecRK1 plants was measured at 1 hour after elicitation with oral secretions of Manduca sexta larvae. Three independent experiments were performed with wild type plants and three independent experiments were performed with ir-lecRK1 plants. A total of 148 genes were identified as differentially regulated in ir-lecRK1 plants compared to wild type plants.

2011-11-23 | E-GEOD-29905 | biostudies-arrayexpress

Dicer-like proteins and their role in plant-herbivore interactions in Nicotiana attenuata

Project description:DICER-like proteins produce small RNAs that silence genes involved in development and defenses against viruses and pathogens. Which DCLs participate in plant-herbivore interactions remains unstudied. We identified four distinct DCL genes and stably silenced their expression by RNAi in Nicotiana attenuata, a model system for the study of plant-herbivore interactions. Silencing DCL1 expression was lethal to the plants. Manduca sexta larvae performed significantly better on ir-dcl3and ir-dcl4 plants, but not on ir-dcl2 plants compared to wild type plants. Phytohormones, defense metabolites and microarray analyses revealed that when DCL3 and DCL4 were silenced separately, herbivore resistance traits were regulated in distinctly different ways. Crossing of the lines revealed complex interactions in the patterns of regulation. Single ir-dcl4 and double ir-dcl2/ ir-dcl3 plants were impaired in JA accumulation, while JA-Ile was increased in ir-dcl3 plants. Ir-dcl3 and ir-dcl4 plants were impaired in nicotine accumulation; silencing DCL2 in combination with either DCL3 or DCL4 restored nicotine levels to those of WT. Trypsin proteinase inhibitor activity and transcripts were only silenced in ir-dcl3 plants. We conclude that DCL2/3/4 interact in a complex manner to regulate anti-herbivore defenses and that these interactions significantly complicate the already challenging task of understanding smRNA function in the regulation of biotic interactions. Gene expression in leaves of Nicotiana attenuata wild type, irDCL3 and irDCL4 plants was measured at 1 hour after elicitation with oral secretions of Manduca sexta larvae. Three independent experiments were performed with wild type plants and three independent experiments were performed with irDCL3 and irDCL4 plants. A total of 811 genes were identified as differentially regulated in irDCL3 and irDCL4 compared to wild type plants.

2013-12-24 | E-GEOD-30124 | biostudies-arrayexpress

Evolution of the herbivore associated elicitor induced early defence signalling networks in Nicotiana

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2016-08-23 | E-GEOD-75006 | biostudies-arrayexpress

NaMYC2 transcription factor regulates a subset of plant defense responses in Nicotiana attenuata

Project description:Identification of significantly altered transcripts in Nicotiana attenuata plants with reduced MYC2 expression after induction with oral secretions of Manduca sexta larvae. Gene expression in leaves of Nicotiana attenuata EV and MYC2_VIGS plants was measured at 1 hour after induction with oral secretions of Manduca sexta larvae. Three independent experiments were performed with EV plants and three independent experiments were performed with MYC2_VIGS plants. A total of 47 genes were identified as differentially regulated in MYC2_VIGS plants compared to EV plants.

2013-05-17 | E-GEOD-45608 | biostudies-arrayexpress

Characterization of Nicotiana attenuata plants with reduced expression of HD-Zip HD20 (ir-hd20 plants).

Project description:Identification of transcripts that change expression in corollas of Nicotiana attenuata plants with reduced expression of the transcription factor HD20. Gene expression in opening corollas of Nicotiana attenuata wild type and ir-hd20 plants was measured from isolated corollas collected at 12 pm. Three independent experiments were performed with wild type plants and three independent experiments were performed with ir-hd20 plants. A total of 274 genes were identified as differentially regulated in ir-hd20 plants compared to wild type plants.

2013-12-24 | E-GEOD-33682 | biostudies-arrayexpress

Characterization of Nicotiana attenuata plants with reduced expression of HSPRO (ir-hspro plants).

Project description:Identification of transcripts that change expression in roots of Nicotiana attenuata plants with reduced expression of HSPRO and in association with Piriformospora indica. Gene expression in roots of Nicotiana attenuata wild type and ir-hspro seedlings was measured at 14 days after inoculation with Piriformospora indica. Three independent experiments were performed with wild type plants and three independent experiments were performed with ir-hspro plants.

2012-11-06 | E-GEOD-35086 | biostudies-arrayexpress

Gene Expression from diabetic ischemic limbs in WT and p75KO mice

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2011-12-24 | E-GEOD-34675 | biostudies-arrayexpress

Transcription profiling of human transiliacal bone biopsies from 7 patients with primary hyperparathyroidism pre and post surgery to investigate the molecular pathology of bone in hyperparathyroidism

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2006-09-04 | E-MEXP-847 | biostudies-arrayexpress

Analysis of the Pseudomonas aeruginosa regulon controlled by the sensor kinase KinB and sigma factor RpoN

Project description:Alginate overproduction by P. aeruginosa, also known as mucoidy is associated with chronic endobronchial infections in cystic fibrosis (CF). Alginate biosynthesis in this bacterium is initiated by the extracytoplasmic function sigma factor (σ22, AlgU/T). In the wild type (wt) nonmucoid strains, such as PAO1, AlgU is sequestered by the anti-sigma factor MucA that inhibits alginate production. However, the degradation of MucA by activated intramembrane proteases AlgW and/or MucP can lead to the conversion from nonmucoid strains to mucoid. Previously we reported that the absence of the sensor kinase KinB in PAO1 causes the initiation of AlgW-dependent proteolysis of MucA resulting in alginate overproduction. In the kinB mutant this activation requires alternate sigma factor RpoN (σ54). To determine the RpoN-dependent KinB regulon, microarray and proteomic analyses were performed on a mucoid kinB mutant and an isogenic nonmucoid kinB rpoN double mutant. In the kinB mutant, RpoN controlled the expression of approximately 20% of the genome. Besides alginate biosynthesis and regulator genes such as AlgW, KinB, in concert with RpoN, also control a large number of genes including: those involved in carbohydrate metabolism, quorum sensing, iron regulation, rhamnolipid production, and motility. In an acute pneumonia murine infection model, mice exhibited better survival when challenged with the kinB mutant than wt PAO1. Together, these data strongly suggest that KinB controls virulence factors important for acute pneumonia and conversion to mucoidy. 6 Samples total. Three with kinB mutant and three kinB wild type.

2012-03-01 | E-GEOD-35248 | biostudies-arrayexpress

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