Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Virus-induced inflammatory gene networks underlying asthma exacerbations in vivo

ABSTRACT: Global patterns of gene expression was profiled in nasal lavage samples obtained from asthmatic children during an acute Picornavirus-induced exacerbation and 7-14 days later. Gene coexpression network analysis and prior knowledge was employed to reconstruct the wiring diagram of the underlying gene networks. The study design consisted of paired samples obtained during the acute exacerbation and 7-14 days later from 16 asthmatic children.

ORGANISM(S): Homo sapiens

SUBMITTER: Anthony Bosco

PROVIDER: E-GEOD-30326 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Interferon regulatory factor 7 is a major hub connecting interferon-mediated responses in virus-induced asthma exacerbations in vivo.

Bosco Anthony A Ehteshami Samira S Panyala Sujatha S Martinez Fernando D FD

The Journal of allergy and clinical immunology 20111123 1

<h4>Background</h4>Exacerbations are responsible for a substantial burden of morbidity and health care use in children with asthma. Most asthma exacerbations are triggered by viral infections; however, the underlying mechanisms have not been systematically investigated.<h4>Objective</h4>The objective of this study was to elucidate the molecular networks that underpin virus-induced exacerbations in asthmatic children in vivo.<h4>Methods</h4>We followed exacerbation-prone asthmatic children prospe ...[more]

PMID: 22112518

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Project description:Background: In asthma, airway epithelium remodeling can already be detected during childhood, and epithelial cells are more susceptible to virus and oxidative stress. Their exact role in natural history and severity of children allergic respiratory disease remains however surprisingly unexplored. Aim: To analyze dysfunctions of epithelium in dust mite allergic respiratory disease (rhinitis ¬± asthma) in children. Methods: Expression profilings of nasal epithelial cells collected by brushing were performed on Affymetrix Hugene 1.0 ST arrays. All allergic patients were sensitized to dust mite. 19 patients had an isolated allergic rhinitis (AR). 14 patients had AR associated with asthma. Patients were compared to 12 controls, their severity and control being assessed according to NAEPP and ARIA criteria. Infections by respiratory viruses were excluded by real-time PCR measurements. Results: 61 probes were able to distinguish allergic rhinitis children from healthy controls. A majority of these probes was under the control of Th2 cytokines, as evidenced by parallel experiments performed on primary cultures of nasal epithelial cells. In uncontrolled asthmatic patients, we observed not only an enhanced expression of these Th2-responsive transcripts, but also a down-regulation of interferon-responsive genes. Conclusion: Our study identifies a Th2 driven epithelial phenotype common to all dust mite allergic children. Besides, it suggests that epithelium is involved in the severity of the disease. Expression profiles observed in uncontrolled asthmatic patients suggest that severity of asthma is linked at the same time to atopy and to impaired viral response. Nasal epithelium gene expression profiling of dust mite allergic children with isolated rhinitis, rhinitis associated with asthma and controls. 38 samples classified in 4 categories : 14 isolated rhinitis (R), 6 rhinitis with uncontrolled asthma (UA), 7 rhinitis with controlled asthma (CA) and 11 healthy subjects (C )

Dataset Information

Virus-induced inflammatory gene networks underlying asthma exacerbations in vivo

Publications

Interferon regulatory factor 7 is a major hub connecting interferon-mediated responses in virus-induced asthma exacerbations in vivo.

Similar Datasets

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