Maintenance of hESCs in mesenchymal stem cell-conditioned media augments hematopoietic specification

ABSTRACT: The realization of human embryonic stem cells (hESC) as a model for human developmental hematopoiesis and potential cell replacement strategies relies on an improved understanding of the extrinsic and intrinsic factors regulating hematopoietic-specific hESC differentiation. Mesenchymal stem cells (hMSCs) are multipotent cells of mesodermal origin that form part of hematopoietic stem cell niches and have an important role in the regulation of hematopoiesis through production of secreted factors and/or cell-to-cell interactions. We have previously shown that hESCs may be successfully maintained feeder-free using hMSC-conditioned media (MSC-CM). Here, we hypothesized that hESCs maintained in MSC-CM may be more prone to differentiation towards hematopoietic lineage than hESCs grown in standard

ORGANISM(S): Homo sapiens

SUBMITTER: Agustin Fernandez 

PROVIDER: E-GEOD-30456 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress