Engineering topology and kinetics of sucrose metabolism in Saccharomyces cerevisiae for improved ethanol yield

ABSTRACT: Sucrose is a major carbon source for industrial bioethanol production by Saccharomyces cerevisiae. In yeasts, two modes of sucrose metabolism occur: (i) extracellular hydrolysis by invertase, followed by uptake and metabolism of glucose and fructose, and (ii) uptake via sucrose-H+ symport followed by intracellular hydrolysis and metabolism. Although alternative start codons in the SUC2 gene enable synthesis of extracellular and intracellular invertase isoforms, sucrose hydrolysis in S. cerevisiae predominantly occurs extracellularly. In anaerobic cultures, intracellular hydrolysis theoretically enables a 9 % higher ethanol yield than extracellular hydrolysis, due to energy costs of sucrose-proton symport. This prediction was tested by engineering the promoter and 5’ coding sequences of SU

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Jean-Marc Daran 

PROVIDER: E-GEOD-30535 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress