RNAi knock down of heterochromatin protein 1 in male and female Drosophila flies

ABSTRACT: Drosophila heterochromatin protein 1- HP1 is believed to be involved in active transcription, transcriptional gene silencing, and the formation of heterochromatin2-7. However, little is known about the function of HP1 during development. Using a Gal4-induced RNA interference system, we show that conditional depletion of HP1 in transgenic flies results in preferential lethality in male flies. Cytological analysis of mitotic chromosomes reveals that HP1 depletion causes sex-biased chromosomal defects, including telomere fusions. The global levels of specific histone modifications, particularly the hallmarks of active chromatin, are preferentially increased in males as well. Expression analysis revealed that approximately twice as many genes are specifically regulated by HP1 in males compared

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Edward Oakeley 

PROVIDER: E-GEOD-3055 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress