Transcriptome sequencing to systematically detect trans-splicing in human embryonic stem cells

ABSTRACT: Trans-splicing occurs post-transcriptionally and generates transcripts that are orderly inconsistent with their corresponding DNA templates. Until recently only exceedingly rare trans-splicing events have been experimentally characterized in the mammalian transcriptomes. Although hundreds to thousands of trans-spliced RNA candidates have been nominated by bioinformatics- or NGS (next-generation sequencing)-based approaches, these candidates unavoidably suffered from potential false positives arising from genetic rearrangement events or in vitro artifacts. Here we develop a pipeline (TSscan) based on NGS transcriptome data to identify trans-splicing in human embryonic stem cells (ESCs). TSscan integrates RNA sequencing data derived from different NGS platforms (i.e., Roche 454, SOLiD, and I

ORGANISM(S): Homo sapiens

SUBMITTER: Chan-Shuo Wu 

PROVIDER: E-GEOD-30557 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress