Transcription profiling of mouse wild-type and B cell-specific TAK1-deficient B cells to elucidate how TAK1 regulates BCR-mediated proliferative response

ABSTRACT: BCR-induced gene expression profile in wild-type and B cell-specific TAK1-deficient B cells; to elucidate how TAK1 regulates BCR-mediated proliferative response Experiment Overall Design: Purified splenic B cells (CD43 negative) were treated with or without anti-IgM (20 micloG/mL) for 4 h. Total RNA was extracted with an RNeasy kit (Qiagen), double-stranded DNA was synthesized from 10 micloG of total RNA with the SuperScript Choice System (Invitrogen) primed with T7-(dT) 24 primer. These cDNAs were used to prepare biotin-labeled cRNA by an in vitro transcription reaction performed using T7 RNA polymerase in the presence of biotinylated-ribonucleotides, according to the manufacturer’s protocol (Enzo Diagnostics). The cRNA product was purified using an RNeasy kit, fragmented, and hybridize

ORGANISM(S): Mus musculus

SUBMITTER: Shizuo Akira 

PROVIDER: E-GEOD-3065 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress