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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Analysis of Gene expression of human Th17 and Th1 clones

ABSTRACT: Human T cell clones were derived according to standard protocols using different mix of cytokines, once assessed for their functional profile were studied for gene expression. The purpose of this study is to analyze human Th1 and Th17 clones with and without stimulation with PMA and Ionomycin to measure differences in gene expression that can distinguish the two lineages. Human T cell clones were derived according to standard protocols using different mix of cytokines, once assessed for their functional profile were studied for gene expression

ORGANISM(S): Homo sapiens

SUBMITTER: Raffaele De Palma

PROVIDER: E-GEOD-30664 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Rarity of human T helper 17 cells is due to retinoic acid orphan receptor-dependent mechanisms that limit their expansion.

Santarlasci Veronica V Maggi Laura L Capone Manuela M Querci Valentina V Beltrame Luca L Cavalieri Duccio D D'Aiuto Elena E Cimaz Rolando R Nebbioso Angela A Liotta Francesco F De Palma Raffaele R Maggi Enrico E Cosmi Lorenzo L Romagnani Sergio S Annunziato Francesco F

Immunity 20120209 2

The reason why CD4(+) T helper 17 (Th17) cells, despite their well-known pathogenic role in chronic inflammatory disorders, are very rare in the inflammatory sites remains unclear. We demonstrate that human Th17 cells exhibit low ability to proliferate and to produce the T cell growth factor interleukin-2 (IL-2), in response to combined CD3 and CD28 stimulation. This was due to the upregulated expression of IL-4-induced gene 1 (IL4I1) mRNA, a secreted L-phenylalanine oxidase, which associated wi ...[more]

PMID: 22326581

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Analysis of Gene expression of human Th17 and Th1 clones

Project description:Human T cell clones were derived according to standard protocols using different mix of cytokines, once assessed for their functional profile were studied for gene expression. The purpose of this study is to analyze human Th1 and Th17 clones with and without stimulation with PMA and Ionomycin to measure differences in gene expression that can distinguish the two lineages.

2012-02-27 | GSE30664 | GEO

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Project description:Maintenance of chromatin structure is essential to eukaryotic life; dysregulation is known to be causal for aberrant development and disease. The Mi-2/nucleosome remodeling and histone deacetylase (NuRD) complex is a multiprotein machine proposed to regulate chromatin structure by nucleosome remodeling and histone deacetylation activities. We identified the localization of MBD3, a component of Mi-2/NuRD complex, in two breast cancer cell lines (MCF7 and MDA-MB-231) using ChIP-Seq. MBD3 showed cell-type specific localization with overlap across cell lines being less than 50%. MBD3 localized across gene bodies, peaking around the transcription start site (TSS). Contrary to existing models, MBD3 preferentially associated with CpG rich promoters marked by H3K4me3. These data suggest that MBD3, and by extension the Mi-2/NuRD complex, may have roles in fine tuning expression for active genes. These data represent an important first step in defining regulatory mechanisms by which Mi-2/NuRD complex controls chromatin structure and gene expression. DamID experiment was performed in human breast cancer cell lines (MCF-7 and MDA-MB-231) in triplicate. Samples were hybridized to NimbleGen Human Whole-Genome Tiling Arrays (0701_HG18_TILE_05_HX1 and 100718_HG18_TILE_05_HX1). MBD3-Dam material was hybridized over Dam-only control.

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DamID for MBD3 in human breast cancer cell lines

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-10-31 | E-GEOD-44776 | biostudies-arrayexpress

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Project description:The aim was to analyze the expression of genes in T cell clones having different functional profiles (Th1, Th0, Th17, Th2) obtained from the same individual. Keywords: Gene expression, T cell clones, Th17 characterisation The T cell clones were derived according to well known protocol, using different cytokines, and classified on the basis of their ability to produce cytokines. Once characterized for their production of IL-4, IL-12, gamma-IFN, IL-13, IL-1, they were used to prepare microarray experiments.

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Expression data of Human Decidual NK cells, Peripheral blood, CD56Bright NK cells and CD56Dim NK cells on HGU133B arrays

Project description:Fragmented RNA cocktails from FACS sorted Human decidual NK cell, and peripheral blood CD56Bright and CD56Dim NK cells, previously hybridization to HGU95AV2 chips (Koopman et al J Exp Med. 2003 Oct 20;198(8):1201-1), were stored long term at -80C, thawed and hybridized to HG-U133B arrays. Transcriptome analysis of Human decidual NK cells and NK cells from peripheral blood using Affymetrix UGU133B arrays.

2016-04-06 | E-GEOD-79939 | biostudies-arrayexpress

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Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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