Insulin-producing cells generated from dedifferentiated human pancreatic beta cells expanded in vitro

ABSTRACT: Expansion of beta cells from the limited number of adult human islet donors is an attractive prospect for increasing cell availability for cell therapy of diabetes. However, while evidence supports the replicative capacity of adult beta cells in vivo, attempts at expanding human islet cells in tissue culture resulted in loss of beta-cell phenotype. Using a genetic lineage-tracing approach we have provided evidence for massive proliferation of beta-cell-derived (BCD) cells within these cultures. Expansion involves dedifferentiation resembling epithelial-mesenchymal transition (EMT). Epigenetic analyses indicate that key beta-cell genes maintain a partially open chromatin structure in expanded BCD cells, although they are not transcribed. Here we report that BCD cells can be induced to redif

ORGANISM(S): Homo sapiens

SUBMITTER: Metsada Pasmanik-Chor 

PROVIDER: E-GEOD-30732 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress