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Transcription profiling time series of S. cerevisiae wild type and upf1 mutant strains following transcription inhibition by thiolutin

ABSTRACT: Isogenic UPF1+ or upf1- yeast strains were treated with 10 ug/ml thiolutin to inhibit global transcription. Targets were obtained from 16 time points: 0, 2, 4, 6, 8, 10, 12, 15, 20, 25, 30, 35, 40, 45, 50, 60 minutes after transcription inhibition. Three biological replicates of each were generated and the expression profiles were determined using Affymetrix YG-S98 arrays. Comparisons between the sample groups allow the identification of genes with differential expression over time between UPF1+ and upf1-. Experiment Overall Design: Sample from 2 genotypes, 16 time points, 3 biological replicates were analyzed

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Michael Culbertson 

PROVIDER: E-GEOD-3076 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Impact of nonsense-mediated mRNA decay on the global expression profile of budding yeast.

Guan Qiaoning Q   Zheng Wei W   Tang Shijie S   Liu Xiaosong X   Zinkel Robert A RA   Tsui Kam-Wah KW   Yandell Brian S BS   Culbertson Michael R MR  

PLoS genetics 20061018 11

Nonsense-mediated mRNA decay (NMD) is a eukaryotic mechanism of RNA surveillance that selectively eliminates aberrant transcripts coding for potentially deleterious proteins. NMD also functions in the normal repertoire of gene expression. In Saccharomyces cerevisiae, hundreds of endogenous RNA Polymerase II transcripts achieve steady-state levels that depend on NMD. For some, the decay rate is directly influenced by NMD (direct targets). For others, abundance is NMD-sensitive but without any eff  ...[more]

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