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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from human corneal endothelial cells

ABSTRACT: Human corneal endothelial cells (HCEC) form a monolayer by adhering tightly through their intercellular adhesion molecules. Located at the posterior corneal surface, they maintain corneal translucency by dehydrating the corneal stroma, mainly through the Na+- and K+-dependent ATPase (Na+/K+-ATPase). Because HCEC proliferative activity is low in vivo,we tried to activate proliferation of HCEC by inhibiting cyclin-dependent kinase inhibitors.We have here demonstrated microarray data of transduced human corneal endothelial cell lines. Affymetrix human U133 plus 2.0 array was used to transcriptionally profile to compare cultured human corneal endothelial cells and transduced human corneal endothelial cells.

ORGANISM(S): Homo sapiens

SUBMITTER: Akihiro Umezawa

PROVIDER: E-GEOD-30781 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression data from human corneal endothelial cells

Project description:Human corneal endothelial cells (HCEC) form a monolayer by adhering tightly through their intercellular adhesion molecules. Located at the posterior corneal surface, they maintain corneal translucency by dehydrating the corneal stroma, mainly through the Na+- and K+-dependent ATPase (Na+/K+-ATPase). Because HCEC proliferative activity is low in vivo,we tried to activate proliferation of HCEC by inhibiting cyclin-dependent kinase inhibitors.We have here demonstrated microarray data of transduced human corneal endothelial cell lines.

2011-10-30 | GSE30781 | GEO

450k Analysis of 24 HNSCC cell lines

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2012-11-30 | E-GEOD-38270 | biostudies-arrayexpress

FF and FFPE HPV+ and HPV- HNSCC and HNSCC cell lines

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2012-11-30 | E-GEOD-38271 | biostudies-arrayexpress

High throughput gene expression analysis identifies reliable expression markers of human corneal endothelial cells.

Project description:Considerable interest has been generated for the development through cell-tissue engineering of suitable corneal endothelial graft alternatives, which can potentially alleviate the shortage of corneal transplant material. The advent of less invasive suture-less key-hole surgery options such as DescemetM-bM-^@M-^Ys Stripping Endothelial Keratoplasty (DSEK) and DescemetM-bM-^@M-^Ys Membrane Endothelial Keratoplasty (DMEK), which involve transplantation of solely the endothelial layer instead of full thickness cornea, provide further impetus for the development of alternative endothelial grafts for clinical applications. A major challenge for this endeavor is the lack of specific markers for this cell type. To identify genes that reliably mark corneal endothelial cells (CECs) in vivo and in vitro, we performed RNA-sequencing on freshly isolated human CECs (from both young and old donors), CEC cultures, and corneal stroma. Gene expression of these corneal cell types were also compared to that of other human tissue types. Based on high throughput comparative gene expression analysis, we identified a panel of markers that are: i) highly expressed in CECs from both young donors and old donors; ii) expressed in CECs in vivo and in vitro; and iii) not expressed in corneal stroma keratocytes and the activated corneal stroma fibroblasts. These were SLC4A11, COL8A2 and CYYR1. The use of this panel of genes in combination reliably ascertains the identity of the CEC cell type. A total of 20 donor corneas consisting of 10 single donor corneas and 5 paired donor corneas were used in this study. Donor age ranged from 19 - 76. This RNA-seq study included 15 pooled corneas (5 each) used form CEC old, CEC young and stroma samples.

2013-05-23 | E-GEOD-44064 | biostudies-arrayexpress

Proinflammatory effect of Pseudomonas aeruginosa hook protein FlgE on human corneal epithelial cells

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2014-10-08 | E-GEOD-58422 | biostudies-arrayexpress

RNA sequencing analysis for comparing gene expression of the corneal endothelium of Slc4a11-/- vs Slc4a11+/+ mice

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Effect of HPV 16 E6, E7 oncoproteins on the expression level of cellular genes

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2014-06-26 | E-GEOD-58841 | biostudies-arrayexpress

Identification of Novel Molecular Markers through Transcriptomic Analysis in Human Fetal and Adult Corneal Endothelial Cells

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2013-01-08 | E-GEOD-41616 | biostudies-arrayexpress

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2011-10-09 | E-GEOD-24981 | biostudies-arrayexpress

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