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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of barley plants (Hordeum vulgare L.) during salinity stress

ABSTRACT: Shoot tissue from 17 day old barley plants was used for expression analysis. Targets from three biological replicates of each were generated and the expression profiles were determined using Affymetrix Barley1 Genechip arrays. Comparisons between the control and stressed samples at three time points (3, 8 and 27 h) allow the identification of salt stress responsive genes. Experiment Overall Design: 3 control and 3 salt stressed biological replicates were analyzed at 3, 8 and 27 h.

ORGANISM(S): Hordeum vulgare

SUBMITTER: Harkamal Walia

PROVIDER: E-GEOD-3097 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression analysis of barley (Hordeum vulgare L.) during salinity stress.

Walia Harkamal H Wilson Clyde C Wahid Abdul A Condamine Pascal P Cui Xinping X Close Timothy J TJ

Functional & integrative genomics 20060201 2

Barley (Hordeum vulgare L.) is a salt-tolerant crop species with considerable economic importance in salinity-affected arid and semiarid regions of the world. In this work, barley cultivar Morex was used for transcriptional profiling during salinity stress using a microarray containing approximately 22,750 probe sets. The experiment was designed to target the early responses of genes to a salinity stress at seedling stage. We found a comparable number of probe sets up-regulated and down-regulate ...[more]

PMID: 16450154

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Barley1 salinity stress study

Project description:Shoot tissue from 17 day old barley plants was used for expression analysis. Targets from three biological replicates of each were generated and the expression profiles were determined using Affymetrix Barley1 Genechip arrays. Comparisons between the control and stressed samples at three time points (3, 8 and 27 h) allow the identification of salt stress responsive genes. Keywords: repeat

2005-11-18 | GSE3097 | GEO

Functional Genomic Analysis Of Barley(Hordeum vulgare L.) Grain Protein Accumulation

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Transcription profiling of barley under salinity stress and JA treatment

Project description:Expression Data of Barley Crown and Growing Point Tissue Under Salt Stress abd JA treatment Experiment Overall Design: Barley genotype Golden Promise was used for expression anlaysis using the tissue from crown and growing point under control, salt stressed, JA treatment and JA pretreatment followed by salt stress

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Array-based genotyping and expression analysis of barley cv. Maythorpe and Golden Promise

Project description:Salt Stress response of salt-tolerant genotype Golden Promise compared to Maythorpe Barley1 GeneChip was used to find differential expression between two barley genotypes under control and salt stress conditions at vegetative stage of growth Keywords: genotype and treatment comparison

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Transcription profiling of barley roots and shoots from the cultivars Maythorpe and Golden Promise grown under normal and high salinity conditions

Project description:Salt Stress response of salt-tolerant genotype Golden Promise compared to Maythorpe; Barley1 GeneChip was used to find differential expression between two barley genotypes under control and salt stress conditions at vegetative stage of growth Experiment Overall Design: Roots (tips) and shoot (crown and growing point) tissue was used for hybridization to GeneChips

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Functional Genomic Analysis Of Barley(Hordeum vulgare L.) Grain Protein Accumulation

Project description:Effect of high grain protein locus on barley grain protein accumulation. Gene expression levels were analysed in Karl, a low grain protein variety with its near-isogenic line 10_11(has high grain protein locus, chromosome 6)using Barley1 22k affymetrix chip. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Aravind Jukanti. The equivalent experiment is BB53 at PLEXdb.] developmental stage: 14 days past anthesis - genotype: Karl - tissue type: Leaves(3-replications); developmental stage: 14 days past anthesis - genotype: Karl - tissue type: Kernels(3-replications); developmental stage: 14 days past anthesis - genotype: 10-11 (NIL with high grain protein locus) - tissue type: Leaves(3-replications); developmental stage: 14 days past anthesis - genotype: 10-11 (NIL with high grain protein locus) - tissue type: Kernels(3-replications); developmental stage: 21 days past anthesis - genotype: Karl - tissue type: Leaves(3-replications); developmental stage: 21 days past anthesis - genotype: Karl - tissue type: Kernels(3-replications); developmental stage: 21 days past anthesis - genotype: 10-11 (NIL with high grain protein locus) - tissue type: Leaves(3-replications); developmental stage: 21 days past anthesis - genotype: 10-11 (NIL with high grain protein locus) - tissue type: Kernels(3-replications)

2011-11-22 | E-GEOD-33413 | biostudies-arrayexpress

Genetic Variation and Alleviation of Salinity Stress in Barley (Hordeum vulgare L.).

Project description: Not available

| S-EPMC6222302 | biostudies-literature

Transcription profiling of barley single feature polymorphisms and drought stress gene expression

Project description:Detection of single feature polymorphisms comparing five barley genotypes. Gene expression under unstressed and drought stressed conditions. Tissue from five entire five day old seedlings from drought stress or unstressed growth conditions was used for RNA extraction. For Barke, Morex and Stepoe the two types of RNA were pooled. For Oregon Wolfe Barley Dominant and Recessive (OWBs), the two types of RNA were handled separately. Targets from three biological replicates of each genotype-treatment were generated and transcript levels were determined using Affymetrix Barley1 GeneChip arrays. Probe set, followed by single probe, comparisons between genotypes allows the identification of single feature polymorphisms in comparisons between genotypes. For the OWBs, comparisons between stressed and unstressed conditions defines stress-regulated genes. Experiment Overall Design: Three genotypes, three replicates each. Two additional genotypes, two sets of three replicates each.

2008-06-12 | E-GEOD-3170 | biostudies-arrayexpress

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