Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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RNA-seq of Pacific oyster Crassostrea gigas under different developmental stages and stress treatments

ABSTRACT: Deep sequencing of samples from different development stages, different adult organs and different stress treatments of Pacific oyster Crassostrea gigas Samples of 38 developmental stages from egg to juvenile were analyzed using single-end 49 bp RNA-seq. Two libraries mixed by RNAs from different developmental stages were analyzed using paired-end 90 bp RNA-seq. A total of 11 samples mainly from 8 organs (mantle, gill,adductor muscle, digestive gland, hemocyte, labial palp, female gonad and male gonad) were analyzed using paired-end 90 bp RNA-seq. At the same time, single-end 49 bp RNA-seq was conducted on 61 samples collected from adult oysters subjected to 9 types of environmental stressors (exposure to air, salinity, temperature, and exposure to metals).

ORGANISM(S): Crassostrea gigas

SUBMITTER: Yabing Zhu 

PROVIDER: E-GEOD-31012 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The oyster genome reveals stress adaptation and complexity of shell formation.

Zhang Guofan G   Fang Xiaodong X   Guo Ximing X   Li Li L   Luo Ruibang R   Xu Fei F   Yang Pengcheng P   Zhang Linlin L   Wang Xiaotong X   Qi Haigang H   Xiong Zhiqiang Z   Que Huayong H   Xie Yinlong Y   Holland Peter W H PW   Paps Jordi J   Zhu Yabing Y   Wu Fucun F   Chen Yuanxin Y   Wang Jiafeng J   Peng Chunfang C   Meng Jie J   Yang Lan L   Liu Jun J   Wen Bo B   Zhang Na N   Huang Zhiyong Z   Zhu Qihui Q   Feng Yue Y   Mount Andrew A   Hedgecock Dennis D   Xu Zhe Z   Liu Yunjie Y   Domazet-Lošo Tomislav T   Du Yishuai Y   Sun Xiaoqing X   Zhang Shoudu S   Liu Binghang B   Cheng Peizhou P   Jiang Xuanting X   Li Juan J   Fan Dingding D   Wang Wei W   Fu Wenjing W   Wang Tong T   Wang Bo B   Zhang Jibiao J   Peng Zhiyu Z   Li Yingxiang Y   Li Na N   Wang Jinpeng J   Chen Maoshan M   He Yan Y   Tan Fengji F   Song Xiaorui X   Zheng Qiumei Q   Huang Ronglian R   Yang Hailong H   Du Xuedi X   Chen Li L   Yang Mei M   Gaffney Patrick M PM   Wang Shan S   Luo Longhai L   She Zhicai Z   Ming Yao Y   Huang Wen W   Zhang Shu S   Huang Baoyu B   Zhang Yong Y   Qu Tao T   Ni Peixiang P   Miao Guoying G   Wang Junyi J   Wang Qiang Q   Steinberg Christian E W CE   Wang Haiyan H   Li Ning N   Qian Lumin L   Zhang Guojie G   Li Yingrui Y   Yang Huanming H   Liu Xiao X   Wang Jian J   Yin Ye Y   Wang Jun J  

Nature 20120919 7418

The Pacific oyster Crassostrea gigas belongs to one of the most species-rich but genomically poorly explored phyla, the Mollusca. Here we report the sequencing and assembly of the oyster genome using short reads and a fosmid-pooling strategy, along with transcriptomes of development and stress response and the proteome of the shell. The oyster genome is highly polymorphic and rich in repetitive sequences, with some transposable elements still actively shaping variation. Transcriptome studies rev  ...[more]

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