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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression analysis of human lymphoblastoid cell lines

ABSTRACT: Human lymphoblastoid cell lines (EBV-immortalised B cells, LcL) obtained from subjects of different age (young 28-40 years, centenarians >95 years) were analysed for gene expression at basal culture conditions and after 48 hours of serum starvation. Lymphoid B cells from centenarians were more resistant to apoptosis induction and displayed a more developed lysosomal compartment, the most critical component of phagic machinery. In addition, cells from centenarians were capable of engulfing and digesting other cells, i.e. their siblings (even entire cells). This behavior was improved by nutrient deprivation but, strikingly, it was unaffected by the autophagy modulating drugs rapamycin, an autophagy inducer, and 3-methyladenine, an autophagy inhibitor. 12 samples, divided into 4 groups: young basal, young starved, centenarians basal and centenarians starved.

ORGANISM(S): Homo sapiens

SUBMITTER: Daniel Remondini

PROVIDER: E-GEOD-31040 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene expression analysis of human lymphoblastoid cell lines

Project description:Human lymphoblastoid cell lines (EBV-immortalised B cells, LcL) obtained from subjects of different age (young 28-40 years, centenarians >95 years) were analysed for gene expression at basal culture conditions and after 48 hours of serum starvation. Lymphoid B cells from centenarians were more resistant to apoptosis induction and displayed a more developed lysosomal compartment, the most critical component of phagic machinery. In addition, cells from centenarians were capable of engulfing and digesting other cells, i.e. their siblings (even entire cells). This behavior was improved by nutrient deprivation, but strikingly, it was unaffected by the autophagy-modulating drugs rapamycin, an autophagy inducer, and 3-methyladenine, an autophagy inhibitor.

2011-07-30 | GSE31040 | GEO

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2014-11-17 | E-GEOD-56932 | biostudies-arrayexpress

Gene Expression Data in Human Monocyte-derived Dendritic Cell (MoDC) from Young and Aged Donors

Project description:Dendritic cells (DCs) are major antigen-presenting cells that play a key role in initiating and regulating innate and adaptive immune responses. DCs are critical mediators of tolerance and immunity. The functional properties of DCs changes with age. The purpose of this study was to define the age-associated molecular changes in DCs by gene array analysis using Affymatrix GeneChips. We identified up and down-regulated gene expression changes in DC from aged donors compared to young donors. Total 9 MoDC RNA samples from four young donors (20-27 years) and five aged donors (77-84 years) were analyzed using Affymetrix HG-U133A_2 Gene Arrays to compare differential gene expression changes in MoDC between aged and young groups

2014-05-28 | E-GEOD-58015 | biostudies-arrayexpress

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2011-08-19 | E-GEOD-31397 | biostudies-arrayexpress

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2021-06-03 | E-MTAB-10413 | biostudies-arrayexpress

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2009-10-22 | E-MEXP-2379 | biostudies-arrayexpress

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2013-10-01 | E-MEXP-3878 | biostudies-arrayexpress

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