DNA methylation alterations and transcriptional gene silencing induced by IDH1 R132H mutation [Affymetrix]

ABSTRACT: The cytosolic NADP+-dependent isocitrate dehydrogenase IDH1 is frequently mutated in human cancers. Recent studies have shown that IDH1 mutant primary glioblastomas (GBM) and acute myeloid leukemias (AML) display robust association with CpG island methylator phenotype (CIMP). Such observations bring into question whether IDH1 mutations directly contribute to the development of CIMP or if the hypermethylation phenotype precedes acquisition of IDH1 mutations. To reveal the effects of IDH1 mutations on DNA methylation and gene expression, we introduced the most frequently observed IDH1 mutation, R132H, into a human cancer cell line through gene targeting. We profiled changes in methylation at over 27,000 CpG dinucleotides spanning 14,475 unique gene regions and characterized genome-wide ge

ORGANISM(S): Homo sapiens

SUBMITTER: Benjamin Barwick 

PROVIDER: E-GEOD-31126 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress