Transcription profiling of human RKO cells after induction of KLF4 for different lengths of time

ABSTRACT: A stably transfected inducible system for KLF4 was established in RKO cells as previously described (Chen et al., 2001; Chen et al., 2003). This cell line, called EcR-RKO/KLF4, contains stably transfected receptors for the insect hormone, ecdysone (EcR), and a full-length mouse KLF4 cDNA driven by a promoter that responds to ecdysone and its analogue, ponasterone A. Cells were treated with 5 µM ponasterone A (PA), for various time durations. To control for the experiment, the vehicle, ethanol, was added for the same periods of time.

ORGANISM(S): Homo sapiens

SUBMITTER: Vincent Yang 

PROVIDER: E-GEOD-3113 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress