Identification of differentially expressed genes in matched primary and metastatic melanoma tumor pairs

ABSTRACT: Comparison of gene expression profiles between a primary melanoma and an early metastatic specimen from the same patient will provide essential biological insight into early metastatic processes. The DASL (cDNA mediated annealing, selection, extension and ligation) assay has been used to generate gene expression data for 502 cancer-related genes from very small formalin-fixed sentinel node biopsy (SNB) melanoma samples, this data has been further compared with gene expression of the matched formalin-fixed primary melanoma. Tissue was sampled from twenty-five SNB deposits using laser capture microdissection. The mean number of genes detected using DASL with SNB samples was lower than when using a core of primary melanoma tumor (242 versus 434 genes). A large proportion of SNB samples failed

ORGANISM(S): Homo sapiens

SUBMITTER: Rosalyn Jewell 

PROVIDER: E-GEOD-31139 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress