Coordinated increase in cellular RNA and protein content induced by overexpression of Far1, a cyclin dependent kinase inhibitor, involves large transcriptional reprogramming and requires the Sfp1 protein.

ABSTRACT: The FAR1 gene encodes a large protein, whose major function is inhibition of cyclin-dependent kinase complexes involved in the G1/S transition. It has been proposed that Far1, together with the G1 cyclin Cln3, may be part of a cell sizer mechanism that controls the entry into S phase. A genome-wide transcriptional analysis of FAR1-overexpressing and far1 deleted cells grown in ethanol- or glucose-supplemented minimal media indicates that FAR1 overexpression induces strong transcriptional remodelling, metabolism being the most affected cellular property. These data suggest that the Far1/Cln3 sizer regulates cell growth either directly or indirectly by affecting metabolism and pathways known to modulate ribosome biogenesis. A crucial role in mediating the effect of Far1 overexpression is pla

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Marco Vanoni 

PROVIDER: E-GEOD-31143 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress