Genomics of Estradiol-3-Sulfate Action in the Ovine Fetal Hypothalamus

ABSTRACT: In the fetal sheep during late gestation sulfoconjugated estrogens in plasma reach a concentration 40-100 times greater than unconjugated estrogens. The objective of the present study was to determine the genomics of estradiol-3-sulfate (E2S) action in the fetal brain. The hypothesis was that E2S stimulates genes involved in the neuroendocrine pathways in the hypothalamus that direct or facilitate fetal development at the end of gestation. Four sets of chronically-catheterized ovine twin fetuses were studied (gestational age: 120-127 days gestation) with one infused with E2S intracerebroventricularly (1 mg/day) and the other remained untreated (control). After euthanasia, mRNA samples were extracted from the 8 hypothalami, corresponding to the four treatment and four control fetuses. Micro

ORGANISM(S): Ovis aries

SUBMITTER: Maria Rabaglino 

PROVIDER: E-GEOD-31184 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress