Differentiation mutants in the protozoan parasite Toxoplasma gondii.

ABSTRACT: Two forms of the protozoan parasite Toxoplasma gondii are associated with intermediate hosts such as humans: rapidly growing tachyzoites are responsible for acute illness, whereas slowly dividing encysted bradyzoites can remain latent within the tissues for the life of the host. In order to identify genetic factors associated with parasite differentiation, we have used a strong bradyzoite-specific promoter (identified by promoter trapping) to drive the expression of T. gondii hypoxanthine-xanthine-guanine phosphoribosyltransferase (HXGPRT) in stable transgenic parasites, providing a stage-specific positive/negative selectable marker. Insertional mutagenesis has been carried out on this parental line, followed by bradyzoite induction in vitro and selection in 6-thioxanthine to identify misr

ORGANISM(S): Toxoplasma gondii

SUBMITTER: Upinder Singh 

PROVIDER: E-GEOD-3119 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress