TCF7 is a key regulator of the switch of self-renewal and differentiation in a multipotential hematopoietic cell line

ABSTRACT: A critical problem in biology is understanding how cells choose between self-renewal and differentiation. To generate a comprehensive view of the mechanisms controlling early hematopoietic precursor self-renewal and differentiation, we used systems-based approaches and murine EML multipotential hematopoietic precursor cells as a primary model. EML cells give rise to a mixture of self-renewing Lin-SCA+CD34+ cells and partially differentiated non-renewing Lin-SCA-CD34- cells in a cell autonomous fashion. We identified and validated the HMG box protein TCF7 as a key regulator in this self-renewal/differentiation switch, and it operates in the absence of canonical Wnt signaling. We found that TCF7 is the most downregulated transcription factor when CD34+ cells switch into CD34- cells using RN

ORGANISM(S): Mus musculus

SUBMITTER: Jiaqian Wu 

PROVIDER: E-GEOD-31221 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress