Bile-inducible membrane protein mediates the bile resistance phenotype of Bifidobacterium breve UCC2003

ABSTRACT: Bbr_0838 from Bifidobacterium breve UCC2003 encodes a 683 residue membrane protein, that contains a permease domain displaying similarity to transporters belonging to the major facilitator superfamily, as well as a CBS (cystathionine beta synthase) domain. The high level of similarity to bile-efflux pumps from other bifidobacteria, suggests a significant role for Bbr_0838 in bile tolerance of B. breve UCC2003. Bbr_0838 transcription was shown to be monocistronic and strongly induced upon exposure to bile. Further analysis delineated the transcriptional start site and the minimal region required for promoter activity and bile regulation. Insertional inactivation of Bbr_0838 in B. breve UCC2003 resulted in a strain that exhibited reduced survival upon cholate exposure as compared to the pare

ORGANISM(S): Bifidobacterium breve UCC2003

SUBMITTER: Aldert Zomer 

PROVIDER: E-GEOD-31224 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress