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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Glutathione-induced elicitation of defense responses in Lotus japonicus.

ABSTRACT: To identify transcription factors involved in the production of defense-related phytochemicals through co-expression studies. The changes in gene expression following GSH elicitation of leaves was followed over a time course. Four biological replicates of the unelicited samples and three biological replicates were used for each time point.

ORGANISM(S): Lotus japonicus

SUBMITTER: Dale Shelton

PROVIDER: E-GEOD-31240 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcription factors of Lotus: regulation of isoflavonoid biosynthesis requires coordinated changes in transcription factor activity.

Shelton Dale D Stranne Maria M Mikkelsen Lisbeth L Pakseresht Nima N Welham Tracey T Hiraka Hideki H Tabata Satoshi S Sato Shusei S Paquette Suzanne S Wang Trevor L TL Martin Cathie C Bailey Paul P

Plant physiology 20120423 2

Isoflavonoids are a class of phenylpropanoids made by legumes, and consumption of dietary isoflavonoids confers benefits to human health. Our aim is to understand the regulation of isoflavonoid biosynthesis. Many studies have shown the importance of transcription factors in regulating the transcription of one or more genes encoding enzymes in phenylpropanoid metabolism. In this study, we coupled bioinformatics and coexpression analysis to identify candidate genes encoding transcription factors i ...[more]

PMID: 22529285

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Constitutive over-expression of LjMyb14 in Lotus japonicus.

Project description:To identify the regulatory targets of the R2R3-Myb transcription factor, LjMyb14, the gene was constitutively over-expressed in Lotus japonicus under the Lotus ubiquitin promoter. The gene expression levels of three biological replicates of the Lotus japonicus (MG20) were averaged and compared to the the gene expression levels of three independent lines of Lotus japonicus japonicus constituitively over expressing LjMyb14 using the Lotus ubiquitin promoter.

2011-09-15 | E-GEOD-31739 | biostudies-arrayexpress

Glutathione-induced elicitation of defense responses in Lotus japonicus.

Project description:To identify transcription factors involved in the production of defense-related phytochemicals through co-expression studies.

2011-09-16 | GSE31240 | GEO

Transcriptome profiling for wild-type, single and double mutants for Nuclear Factor YA1 (nf-ya1) and hypernodulation phenotype (har1) mutants in roots of Lotus japonicus

Project description:Acting in a partially redundant manner, NF-Ys were shown previously to regulate bacterial infection, including selection of a superior rhizobial strain, and to participate in mediating nodule structure formation. However, the exact mechanism(s) by which these transcriptional factors exert their symbiotic functions has remained elusive. Gene expression profiling for wild-type and Nuclear Factor YA (nf-ya)-A mutants in roots of Lotus japonicus were conducted 4 days after inoculation with Mesorhizobium loti in order to understand the interaction of NF-Ys and other genes in Lotus japonicus.

2017-05-03 | E-MTAB-4970 | biostudies-arrayexpress

Cell-type specific transcriptome analysis in nodules of Lotus japonicus

Project description:Legume plants can establish symbiotic nitrogen fixation (SNF) with rhizobia mostly in root nodules, where rhizobia-infected cells are accompanied with uninfected cells in a mosaic pattern. Inside the mature nodules of legume, carbon and nitrogen nutrients between host plant cells and their resident bacteria are actively exchanged. To elucidate the metabolite dynamics relevant for SNF in nodules, three cell-types from nodule tissues of a model legume, Lotus japonicus, were isolated using laser microdissesction, and transcriptome analysis was done by an oligoarray with 60-mer length representing 21,495 genes. In our cell-type-specific profiling, many genes were identified as being expressed in nodules with spatial-specific manners. Among them, genes coding for metabolic enzymes were classified according to their function, and detailed data analysis figured out that secondary metabolic pathway was highly activated in nodule cortex. In particular, a number of metabolic genes for phenyl propanoid pathway were found as highly expressed genes accompanied with those encoding putative transporters of secondary metabolites. These data suggest the involvement of novel physiological function of phenylpropanoids in SNF. Gene expression in three different cell-types of Lotus japonicus nodule was measured. Three independent experiments were performed at each cell-types.

2011-12-29 | E-GEOD-34753 | biostudies-arrayexpress

Transcription profiling of Lotus japonicus

Project description:Transcript profiling of mutant genotypes, treatment responses and organs

2009-07-01 | E-TABM-715 | biostudies-arrayexpress

Transcription profiling of Lotus japonicus major organ systems including nodule and seed development

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Transcription profiling of Lotus japonicus grown under different salt acclimation regimes

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2008-07-17 | E-MEXP-1204 | biostudies-arrayexpress

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2022-03-01 | PXD001964 | Pride

Transcription profiling of Lotus japonicus grown under different nitrogen sources

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2016-05-17 | E-MTAB-4177 | biostudies-arrayexpress

Lotus japonicus-Alkaline

Project description:Lotus japonicus is a model legume broadly used to study transcriptome regulation under different stress conditions and microorganism interaction. Understanding how this model plant respond gainst alkaline stress will certainly help to develop more tolerant cultivars in economically important Lotus species as well as in other legumes. In order to uncover the most important response mechanisms activated during alkaline stress, we explored by microarray analysis the transcriptome regulation occurring in the phenotypically contrasting ecotypes MG-20 and Gifu B-129 of L. japonicus after 21 days of alkaline stress.

2014-03-25 | E-MTAB-2418 | biostudies-arrayexpress

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