Isolation and in vitro expansion of human colonic stem cells [Expression profile]

ABSTRACT: Using the surface marker EPHB2, we have FACS-purified and profiled stem cell-enriched cell fractions from normal human mucosa, crypt proliferative progenitors and late transient amplifying cells to define a gene expression program specific for normal human colon epithelial stem cells We FACS purified human colonic crypt cells according to their EPHB2 surface abundance. We used Affymetrix chips to hybridize 3 samples from EPHB2-high, 3 samples from EPHB2-medium, 3 samples from EPHB2-low, and 2 samples from EPHB2-negative cells

ORGANISM(S): Homo sapiens

SUBMITTER: Eduard Batlle 

PROVIDER: E-GEOD-31255 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress