Transcription profiling of wild type mouse livers and livers from hepatocyte nuclear factor 4alpha deficient mice to study the role of HNF4a in hepatogenesis.

ABSTRACT: To study the role of hepatic nuclear factor _ alpha (HNF4a_ in hepatogenesis, we used loxP-Cre technology to eliminate it from developing mouse livers. A comparison of control and experimental livers revealed that hepatocytes lacking HNF4a_failed to fully differentiate. Specifically, HNF4a null liver cells failed to express genes that are markers of mature hepatocytes including Gys2, Pck1, and G6pc. These cells also failed to form normal cellular junctions, which are critical for establishment of the hepatic epithelium [Parviz et al. (2003) Nat.Genet. 34, 292-96]. Because HNF4a functions as a transcription factor, we hypothesized that it regulates liver development by controlling the expression of genes whose products are essential in executing cellular differentiation and morphogenes

ORGANISM(S): Mus musculus

SUBMITTER: Stephen Duncan 

PROVIDER: E-GEOD-3126 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress