Abnormal Wnt and PI3Kinase signaling in the malformed intestine of lama5 deficient mice

ABSTRACT: Laminins are major constituents of basement membranes and are essential for tissue homeostasis. Laminin-511 is highly expressed in the intestine and its absence causes severe malformation of the intestine and embryonic lethality. To understand the mechanistic role of laminin-511 in tissue homeostasis, we used RNA profiling of embryonic intestinal tissue of lama5 knock out mice combined with cell culture experiments and siRNA mediated knock down and identified a lama5 specific gene expression signature. Our data provide a mechanistic link between laminin alpha5 gene deficiency and the physiological phenotype. We show that laminin alpha5 plays a crucial role in both epithelial and mesenchymal cell behavior by regulating Wnt and PI3K signaling. We provide evidence that a laminin-511 substratu

ORGANISM(S): Mus musculus

SUBMITTER: Bernard Jost 

PROVIDER: E-GEOD-31334 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress