Human ART culture conditions prime mouse embryos for postimplantation development

ABSTRACT: With the increasing use of Assisted Reproductive Technologies (ART) for treatment of human infertility, there is an increasing requirement for embryo culture conditions that perform as similar to nature as possible. How good the match, however, cannot be tested experimentally in human. We solved the central question of how well ART culture protocols prepare embryos for postimplantation development, under the provisions of the 'mouse embryo assay' (MEA). Our side-by-side comparison of 8 conditions [i.e., 3 culture conditions (KSOM, HTF and ISDM1) plus the in vivo system in two different mouse strains (B6 and CD1)] shows that mouse embryos cultured under ART conditions are differentially primed for postimplantation development, and that certain ART protocols outperform the oviduct. The di

ORGANISM(S): Mus musculus

SUBMITTER: Marcos Araúzo-Bravo 

PROVIDER: E-GEOD-31414 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress