Mammary stem cells: molecular profiling to identify novel biomarkers and the stem cell niche

ABSTRACT: Identification and transcriptome analysis of mammary stem cells (MaSC) are important steps toward understanding the molecular and cellular bases of mammary epithelial growth, homeostasis and tissue repair. We identified MaSC based upon their ability to retain bromodeoxyuridine (BrdU) for an extended period. Using laser microdissection, we isolated four categories of cells from the mammary epithelium of female bovine calves. These categories included label retaining epithelial cells (LREC) from the basal layer (LRECb), basal control cells (ECb), LREC from embedded layers (LRECe) and embedded control cells (ECe). Microarray analysis revealed 592 genes that were differentially expressed between LRECb and ECb, and 110 genes between LRECe and ECe. Our analysis supported classification of LRECb

ORGANISM(S): Bos taurus

SUBMITTER: Anthony Capuco 

PROVIDER: E-GEOD-31541 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress