ECAT15-2/Dppa2 transgenic rescue into ECAT15-2/Dppa2 deficient ESCs

ABSTRACT: Many transcription factors and DNA binding proteins play essential roles in the development of organs in which they are highly and/or specifically expressed. Embryonic stem cell (ESC) associated transcript (ECAT) 15-1 and ECAT15-2, also known as developmental pluripotency-associated (Dppa) 4 and Dppa2, are enriched in mouse ESCs and pre-implantation embryos, and encode homologous proteins with a common DNA binding domain known as the SAP motif. Previously, ECAT15-1 was shown to be important in the lung development, while it is dispensable in early development. In this study, we generated ECAT15-2 single and ECAT15-1/15-2 double knockout mice and found that almost all mutants, like ECAT15-1 mutants, died around birth with respiratory defects. Paradoxically, the expression of neither ECAT15-

ORGANISM(S): Mus musculus

SUBMITTER: Shinya Yamanaka 

PROVIDER: E-GEOD-31582 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress