Angus PRKG2 R678X homozygous (dwarf) cattle vs unaffected growth plate cartilage

ABSTRACT: Kinase activity of cGMP-dependant, type II, protein kinase (PRKG2) is required for the proliferative to hypertrophic growth transition of growth plate chondrocytes during endochondral ossification. Loss of PRKG2 function in rodent and bovine models results in dwarfism. We compared growth plate cartilage gene expression profiles of PRKG2R678X/R678X, dwarf and PRKG2R678X/+, unaffected Angus cattle using microarray technology to discover pathways regulated by PRKG2. Calves used in this analysis were produced by the mating between a dwarf carrier sire and two dams- a mother (carrier) and daughter (dwarf) pair. Each dam produced three calves by embryo transfer, which were born in two calving groups. Growth plate cartilage was harvested from the tibia of each animal at approximately 215 days

ORGANISM(S): Bos taurus

SUBMITTER: James Reecy 

PROVIDER: E-GEOD-31627 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress