Gene expression profiles of Calu-3 lung epithelial cells after neutrophil transmigration

ABSTRACT: Injury to the epithelium is integral to the pathogenesis of many inflammatory lung diseases, and epithelial repair is a critical determinant of clinical outcome. However, the signaling pathways regulating such repair are incompletely understood. Herein, we used in vitro and in vivo models to define these pathways. Human neutrophils were induced to transmigrate across monolayers of human lung epithelial cells in the physiologic basolateral-to-apical direction to a gradient of fMLP. Control wells were treated with fMLP alone. Indicated wells were pretreated with an anti-CD47 blocking antibody, which exacerbates epithelial injury. This allowed study of the neutrophil contribution not only to the initial epithelial injury, but also to its repair, as manifest by restoration of transepithelial

ORGANISM(S): Homo sapiens

SUBMITTER: Rachel Zemans 

PROVIDER: E-GEOD-31697 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress