Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

21 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Constitutive over-expression of LjMyb14 in Lotus japonicus.

ABSTRACT: To identify the regulatory targets of the R2R3-Myb transcription factor, LjMyb14, the gene was constitutively over-expressed in Lotus japonicus under the Lotus ubiquitin promoter. The gene expression levels of three biological replicates of the Lotus japonicus (MG20) were averaged and compared to the the gene expression levels of three independent lines of Lotus japonicus japonicus constituitively over expressing LjMyb14 using the Lotus ubiquitin promoter.

ORGANISM(S): Lotus japonicus

SUBMITTER: Dale Shelton

PROVIDER: E-GEOD-31739 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Constitutive over-expression of LjMyb14 in Lotus japonicus.

Project description:To identify the regulatory targets of the R2R3-Myb transcription factor, LjMyb14, the gene was constitutively over-expressed in Lotus japonicus under the Lotus ubiquitin promoter.

2011-09-16 | GSE31739 | GEO

Transcriptome profiling for wild-type, single and double mutants for Nuclear Factor YA1 (nf-ya1) and hypernodulation phenotype (har1) mutants in roots of Lotus japonicus

Project description:Acting in a partially redundant manner, NF-Ys were shown previously to regulate bacterial infection, including selection of a superior rhizobial strain, and to participate in mediating nodule structure formation. However, the exact mechanism(s) by which these transcriptional factors exert their symbiotic functions has remained elusive. Gene expression profiling for wild-type and Nuclear Factor YA (nf-ya)-A mutants in roots of Lotus japonicus were conducted 4 days after inoculation with Mesorhizobium loti in order to understand the interaction of NF-Ys and other genes in Lotus japonicus.

2017-05-03 | E-MTAB-4970 | biostudies-arrayexpress

Cell-type specific transcriptome analysis in nodules of Lotus japonicus

Project description:Legume plants can establish symbiotic nitrogen fixation (SNF) with rhizobia mostly in root nodules, where rhizobia-infected cells are accompanied with uninfected cells in a mosaic pattern. Inside the mature nodules of legume, carbon and nitrogen nutrients between host plant cells and their resident bacteria are actively exchanged. To elucidate the metabolite dynamics relevant for SNF in nodules, three cell-types from nodule tissues of a model legume, Lotus japonicus, were isolated using laser microdissesction, and transcriptome analysis was done by an oligoarray with 60-mer length representing 21,495 genes. In our cell-type-specific profiling, many genes were identified as being expressed in nodules with spatial-specific manners. Among them, genes coding for metabolic enzymes were classified according to their function, and detailed data analysis figured out that secondary metabolic pathway was highly activated in nodule cortex. In particular, a number of metabolic genes for phenyl propanoid pathway were found as highly expressed genes accompanied with those encoding putative transporters of secondary metabolites. These data suggest the involvement of novel physiological function of phenylpropanoids in SNF. Gene expression in three different cell-types of Lotus japonicus nodule was measured. Three independent experiments were performed at each cell-types.

2011-12-29 | E-GEOD-34753 | biostudies-arrayexpress

Transcription profiling of Lotus japonicus major organ systems including nodule and seed development

Project description:Lotus japonicus is a perennial legume with a small diploid genome that has been adopted as a model species for legume genetics and genomics. With the genome sequence as a backdrop (Sato et al. 2008), we have generated a gene expression atlas that provides a global view of gene expression in all major organ systems of this species, including nodule and seed development.

2010-12-22 | E-MEXP-1726 | biostudies-arrayexpress

Transcription profiling of Lotus japonicus grown under different salt acclimation regimes

Project description:Model legume Lotus japonicus was subjected to non-lethal long-term salinity and profiled at the transcriptomic level. Three independent experiments were performed, testing two experimental designs: a traditional gradual acclimation following a step-wise increase of salt concentration and an initial acclimation approach (ia).

2008-07-17 | E-MEXP-1204 | biostudies-arrayexpress

Lotus japonicus-Alkaline

Project description:Lotus japonicus is a model legume broadly used to study transcriptome regulation under different stress conditions and microorganism interaction. Understanding how this model plant respond gainst alkaline stress will certainly help to develop more tolerant cultivars in economically important Lotus species as well as in other legumes. In order to uncover the most important response mechanisms activated during alkaline stress, we explored by microarray analysis the transcriptome regulation occurring in the phenotypically contrasting ecotypes MG-20 and Gifu B-129 of L. japonicus after 21 days of alkaline stress.

2014-03-25 | E-MTAB-2418 | biostudies-arrayexpress

Glutathione-induced elicitation of defense responses in Lotus japonicus.

Project description:To identify transcription factors involved in the production of defense-related phytochemicals through co-expression studies. The changes in gene expression following GSH elicitation of leaves was followed over a time course. Four biological replicates of the unelicited samples and three biological replicates were used for each time point.

2011-09-15 | E-GEOD-31240 | biostudies-arrayexpress

Comparative transcriptomic analysis of Lotus japonicus leves and roots under symbiotic and non-symbiotic conditions.

Project description:Microarray transcriptomic analysis was carried out on Lotus japonicus plants grown either under purely symbiotic conditions (Mesorhizobium loti) or under non-symbiotic conditions (no inoculation and provided with NH4NO3).

2020-07-02 | E-MTAB-8235 | biostudies-arrayexpress

Transcription profiling by array of Lotus japonicus leaves in response to treatment with the nitrification inhibitor DMPP (3,4-dimethylpirazole phosphate)

Project description:Transcriptomic profiling was carried out for leaves of Lotus japonicus plants grown in the presence or absence of 10 mg/L-1 of the nitrification inhibitor DMPP (3,4-dimethylpirazole phosphate).

2019-07-01 | E-MTAB-7268 | biostudies-arrayexpress

Transcription profiling of Lotus japonicus grown under different nitrogen sources

Project description:Transcriptomic profiling was carried out for leaves of Lotus japonicus plants grown with different mineral nitrogen sources (NO3-, NH4+ or NH4NO3) or under conditions of biological nitrogen fixation (Nod). Nodulated plants were inoculated with Mesorhizobium loti and watered with nitrogen-free âHornumâ medium supplemented with 3 mM KCl. Plants under different nitrogen nutritions were watered with âHornumâ nutrient solution containing 10 mM KNO3 (NO3- plants) or with 10 mM NH4Cl supplemented with 3 mM KCl (NH4+ plants) or with 5 mM NH4NO3 supplemented with 3 mM KNO3 (NH4NO3 plants). After all the plants reached the size of 7 trifoils, leaf tissue was harvested. Every harvest involved at least three independent biological replicates for each treatment.

2016-05-17 | E-MTAB-4177 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data